Determination of cell viability using acridine orange/propidium iodide dual-spectrofluorometry assay
نویسندگان
چکیده
منابع مشابه
Dual Effects of Plant Antioxidants on Neuron Cell Viability
Background : Many studies have focused on oxidative stress induced damage and hence, the protective effects conferred by antioxidants. An example is neurodegenerative diseases which is thought to occur due to neuronal loss associated with oxidative stress. However, some antioxidants such as vitamin E have been shown to also exert pro-oxidative effects at high concentration. Objective : In t...
متن کاملDetermination of Metabolic Viability and Cell Mass Using a Tandem Resazurin/Sulforhodamine B Assay.
The identification of rapid, reliable, and highly reproducible biological assays that can be standardized and routinely used in preclinical tests constitutes a promising approach to reducing drug discovery costs and time. This unit details a tandem, rapid, and reliable cell viability method for preliminary screening of chemical compounds. This assay measures metabolic activity and cell mass in ...
متن کاملDual DNA staining assessment of bovine sperm viability using SYBR-14 and propidium iodide.
A new membrane-permeant DNA stain, SYBR-14, was used in combination with propidium iodide (PI) to estimate the proportion of living sperm in bovine semen. The SYBR-14 stained living sperm while PI only stained degenerate cells that had lost their membrane integrity. Staining with SYBR-14 resulted in the nuclei of living sperm fluorescing bright green. Aliquots containing nearly all living bovin...
متن کاملTitration of non-occluded baculovirus using a cell viability assay.
system: synthesis of antibacterial peptide cecropin. FEBS Lett. 414:268-270. 5.Nemetz, C., S. Wessner, R. Schweizer, M. Watzele, T. Metzler, W. Mutter, and B. Buchberger. 2002. RTS: rapid protein expression directly from PCR fragments. Biochemica 2:22-24. 6.Sauer, B. 1994. Site-specific recombination: developments and applications. Curr. Opin. Biotechnol. 5:521-527. 7.Spirin, A.S., V.I. Baranov...
متن کاملEvaluating the Effects of Dual Frequency Sonication Parameters on Acoustic Cavitation by Chemical Dosimeter Using Iodide
Background and Aims: Production of acoustic cavitation by sonication has been recently recommended as a targeted treatment. The experimental results from studies indicate that the activity of cavitation generated by bi- or multi-frequency ultrasound irradiation is higher than that caused by single frequency irradiation. In this study, effects dual (1 MHz and 40 kHz) and single frequency soni...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Cogent Food & Agriculture
سال: 2019
ISSN: 2331-1932
DOI: 10.1080/23311932.2019.1582398